Holotomography is a groundbreaking technology that enables high-resolution observation of dynamic changes in cells without fluorescent staining, while preserving their natural state. This technique does not interfere with the physiological changes of cells, allowing for real-time analysis of their internal structures and functions over extended periods.
- LEE (SNU): “Our research team utilized holotomography to observe various dynamic changes, such as cell division, migration, and death, in mouse intestinal organoids over a period of approximately 120 hours. Without fluorescent staining, we analyzed the internal structures of cells with a high resolution of 155 nm and successfully tracked toxicity and efficacy changes following cisplatin treatment in real time. This demonstrates holotomography’s exceptional capability for long-term observation and precise analysis, which is difficult to achieve with conventional technologies.”
- KONG (IBS):Â “We combined iPSC-derived blood vessel organoids (BVOs) with holotomography to implement an atherosclerosis model and utilize it as a drug screening platform. BVOs possess a 3D structure and multicellular environment, enabling the replication of cell-to-cell interactions that are challenging to observe in 2D models. With holotomography, we non-invasively observed dynamic processes such as foam cell formation and inflammatory responses within the organoids and tracked these in real time.”
Holotomography is playing a pivotal role in overcoming the limitations of traditional imaging technologies in various studies, particularly in disease modeling and drug screening. Its non-invasive nature and high-resolution imaging capabilities open up new possibilities for drug development and disease research.