Traditional microscopy methods often require fluorescent labeling to analyze cellular structures, which can be time-consuming and invasive. In contrast, our HT-X1 system allows for high-resolution visualization of cellular morphology without the need for any labeling, offering a clear advantage in live-cell imaging. To validate this capability, we conducted an organoid-based analysis using intestinal organoids.
Among various organoid types, intestinal organoids are known to closely replicate the functional structure of the gut, comprising intestinal stem cells, enterocytes, tuft cells, goblet cells, and cytoskeletal components.
In this study, we induced Leaky Gut Syndrome (LGS) by treating the intestinal organoids with LPS and then applied candidate therapeutic compounds. The morphological changes related to epithelial barrier recovery were captured and quantified using the HT-X1 system.
Furthermore, we evaluated the real-time adhesion of probiotic strains to the intestinal epithelial cells, enabling the functional characterization of the epithelial layer. This approach supported disease modeling and efficacy testing, particularly for inflammatory bowel disease (IBD) and autoimmune-related conditions.
Overall, HT-X1 demonstrated its capability as a non-invasive, label-free imaging solution for analyzing complex 3D cell models. It holds strong potential as a next-generation platform for drug efficacy screening and functional cellular analysis, especially in advanced organoid-based research