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Immune Cell

Macrophage

Macrophage_main

  • A co-culturing platform of macrophages and organoids demonstrates varying organoid cytotoxic effects depending on the ratio of M1 macrophages to M2 macrophages.
  • Optimal cell death effects are established by co-culturing different ratios of functionally diverse immune cells.
  • This enables the identification of optimal conditions for co-culturing various immune cells, facilitating the selection of an appropriate platform for drug testing.

Organism
Human
Product Type
Organoid + T cell + Macrophage
Tissue
Innate
Disease

Applications

Colorectal cancer

Colorectal cancer organoids faithfully mimic patient tumors, aiding drug testing and personalized treatment strategies through biomarker identification and high-throughput screening in a realistic tumor microenvironment

Non-small cell lung cancer

Non-small cell lung cancer organoids mirror patient tumor diversity and genetics, providing a robust platform for detailed cancer research, including drug responses and personalized treatment exploration

Pancreatic cancer

Pancreatic cancer organoids replicate patient tumor complexity, informing drug responses, disease modeling, and biomarker discovery to advance personalized treatment strategies and research

Breast cancer

Patient-derived breast cancer organoids mimic tumor complexities, facilitating diverse drug testing and precision medicine research for personalized treatment strategies

Cholangiocarcinoma

Cholangiocarcinoma organoids aid in personalized treatment strategies by replicating patient tumor complexity and advancing research through biomarker identification and drug screening

Table of Contents

Characteristics

The co-culture drug evaluation solution with macrophages, T cells, and cancer organoids allows for the simultaneous observation of the phagocytic activity of macrophages and the cytotoxic ability of T cells.
Co-culturing with both M1 and M2 macrophages is possible, mimicking the role of macrophages observed in actual cancer tissues.

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Process

By utilizing patient-derived tumor organoids and T cells, macrophages, this is a new evaluation solution with mechanisms involving two or more immune cells.
Generally, M1 macrophages are known to be associated with tumor growth suppression and M2 macrophages are known to be associated with tumor growth promotion.
To perform different functions, they have different immune markers, metabolic characteristics and gene profiles.
In vivo, proper balance of differentiation into M1 and M2 macrophages is required to elicit an immune response against tumors.
For the development of a new drug evaluation solution, we conducted efficacy evaluations of drugs and antibodies on co-cultures of M1 macrophages, M2 macrophages and tumor organoids.
After conducting the efficacy evaluation, growth rates and mortality were measured to establish optimal co-culture conditions

O : M1 : M2

To enable efficient testing, we have developed an evaluation solution utilizing macrophages derived from pluripotent stem cells.
Ultimately, we have created an optimal co-culture model with macrophages derived from pluripotent stem cells, T cells and tumor organoids. By testing various drug conditions on this model, we confirmed high drug response rates.
We have selected these rates as evaluation criteria for immuno-oncology drugs.
I suggest challenging more advanced research through this new evaluation solution involving multiple immune cells.
It could be a more effective strategy for your research.

PSC_Macrophage