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Organism | |
Product Type | Organoid |
Tissue | iPSC |
Disease |
Applications
Efficacy assay
Organoids are three-dimensional mini organs that mimic the functions of respective organs, reproducing their complex physiological structures and functions. Verify their efficacy with our advanced technology.
Toxicity test
Organoids are three-dimensional mini organs that mimic the functions of respective organs, reproducing their complex physiological structures and functions. Assess their toxicity with our advanced technology!
Leverage our breakthrough cardiac organoids, engineered from human-induced pluripotent stem cells (iPSCs).
By transforming 3D embryoid bodies through precise signal manipulation, we create organoids that emulate heart tissue, advancing personalized cardiac research and therapy.
To create cardiac organoids, we utilized Wnt signaling activators and inhibitors, which are crucial for heart development, at various stages of differentiation within embryoid bodies.
By day eight of differentiation, we observed spontaneous beating in the organoids.
The cardiac organoids produced are composed similarly to the human heart, containing cardiomyocytes and non-muscle cells like cardiac fibroblasts and endothelial cells.
Through FACS analysis, it was determined that cardiomyocytes constitute about 65% of the cell population, with the majority of the non-muscle cells being fibroblasts and endothelial cells.
Using immunofluorescence, we have confirmed the expression of myocardial markers (cTnT, α-actinin), fibroblast markers (α-SMA), and endothelial cell markers (CD31) in our cardiac organoids.
This method allows for detailed visualization and verification of specific cellular components critical to heart tissue function.
To analyze the electro physiological properties of cardiac organoids with spontaneous beating, we employ the patch clamp technique. By inserting electrodes into the cell membranes of the cardiac organoids, we can monitor action potentials in real-time, providing valuable insights into their functional behavior.
Cardiac organoids have electrophysiological properties similiar to cardiac tissue
@ 2024 . All rights reserved
@ 2024 . All rights reserved
