Adaptive Spatial Profiling Solution
OPAL has the advantage of being able to stain freely without restrictions on the host of the primary antibody, making it easy to use antibodies already available in the laboratory and increasing experimental flexibility.
It is possible to stain up to 8 biomarkers at a time, and has the advantage of being relatively cheaper than CODEX staining.
Staining
2 days
Image
1-3 days per slide
Analyze
4 months
Liver organoid
H&E
OPAL
Merge
DAPI
ALB
ALB
PDGFR
BSEP
COL1
CD31
Brain organoid
H&E
OPAL
Merge
DAPI
NeuN
NF-M
MAP2
GFAP
SOX2
NG2
Colon organoid
H&E
OPAL
Merge
DAPI
ChaA
ECAD
SOX9
Lyz
Muc2
SI
Confirmation of similarity using OPAL staining in organoids derived from various organs undergoing differentiation.
OPAL
Merge
DAPI
OLFM4
SLC26A3
MUC2
FCGBP
KRT20
TMM40
Outcomes of organoid and co-culture cell analysis through OPAL staining
