The Hair Growth Efficacy Assay (3D) is a sophisticated method conducted with hair follicle organoids, enabling precise evaluation of hair growth efficacy. In Lambda, our platform excels in skin organoid models, ensuring consistent shape and hair follicle formation.
An Anti-alopecia Efficacy Assay conducted in a three-dimensional (3D) culture is designed to evaluate the effectiveness of substances or treatments in preventing or treating hair loss (alopecia). Lambda provides a solution by utilizing highly biomimetic hair follicle organoids to more accurately mimic cell-to-cell interactions, enabling the assessment of drug effects and development.
An Anti-alopecia efficacy assay conducted in a three-dimensional (3D) culture is designed to evaluate the effectiveness of substances or treatments in preventing or treating hair loss (alopecia). Lambda provides a solution by utilizing highly biomimetic hair follicle organoids to more accurately mimic cell-to-cell interactions, enabling the assessment of drug effects and development.
Assay process
Step 1: Stabilizing induced pluripotent stem cells after culturing for the differentiation of skin organoids.
Step 2: Cells are cultured on a U-bottom plate to form embryoid bodies (EBs) and differentiate into skin organoids.
Step 3: Treatment of male hormone (DHT) alone or simultaneously with the sample before checking the hair placode.
Step 4: Compare the number of hair follicles formed in the experimental group treated with DHT alone and the experimental group treated simultaneously with the sample.
Identify markers related to skin structure and hair follicles through Immunofluorescence (IF) analysis to evaluate the hair loss efficacy of the sample.
Technical
Histological analysis
Hair follicle counting
Morphology analysis
Observe the morphology of the group treated with DHT alone and the group treated simultaneously with hair root strengthening substances Minoxidil and SOYACT. In the simultaneously administered group, more hair follicles were observed compared to the group administered DHT alone.
Immunofluorescence
The Immunofluorescence (IF) results in the group treated simultaneously with DHT and Minoxidil, DHT and SOYACT confirmed an increase in SOX2.