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Skin & Hair

Lambda’s hair follicle organoid, characterized by high reliability, can be utilized for drug development.

Starting cost
0 +

Hair

€4.000+

The Hair Growth Efficacy Assay (3D) is a sophisticated method conducted with hair follicle organoids, enabling precise evaluation of hair growth efficacy.
In Lambda, our platform excels in skin organoid models, ensuring consistent shape and hair follicle formation.

Cell Type

· iPSC

Organoid type

· Skin & Hair folicle organoid

An Anti-alopecia Efficacy Assay conducted in a three-dimensional (3D) culture is designed to evaluate the effectiveness of substances or treatments in preventing or treating hair loss (alopecia).
Lambda provides a solution by utilizing highly biomimetic hair follicle organoids to more accurately mimic cell-to-cell interactions, enabling the assessment of drug effects and development.

Cell Type

· iPSC

Organoid type

· Skin & Hair folicle organoid

Assay

Hair growth efficacy assay (3D)

The Hair growth efficacy assay (3D) is a sophisticated method conducted with hair follicle organoids, enabling precise evaluation of hair growth efficacy.
In Lambda, our platform excels in skin organoid models, ensuring consistent shape and hair follicle formation.
Through a meticulous comparison of hair follicle numbers and Immunofluorescence (IF) analysis, Lambda evaluates hair growth efficacy by confirming markers related to skin and follicle structures. These advancements in skin organoids present Lambda as a solution for precisely assessing hair regrowth efficacy.

Due to the ban on animal testing in cosmetics by the EU in 2013 and South Korea in 2017,
there’s an increasing need to overcome the limitations of current efficacy and safety testing methods.
Lack of accurate efficacy evaluation for escpecially hair product .

Offering more accurate hair care product testing
  • Unique testing methods enabled by our skin organoids, including hair follicle and nerve cell structures.
  • Our skin organoid technology mimics the epidermal, dermal, and subcutaneous fat layers found in human skin.
  • Replicating human skin’s complexity.

Assay process

  • Step 1: Stabilizing induced pluripotent stem cells after culturing for the differentiation of skin organoids.
  • Step 2: Cells are cultured on a U-bottom plate to form embryoid bodies (EBs) and differentiate into skin organoids.
  • Step 3: Treat samples before identifying hair placodes.
  • Step 4: Evaluate the efficacy of hair growth in the sample by comparing the number of hair follicles in the control group and the treated group, and confirm markers related to skin structure and hair follicles through Immunofluorescence (IF) analysis.

Technical

Hair follicle counting

As a result of comparing the number of hair follicles after treating hair growth efficacy drugs, it was confirmed that the number of hair follicles was higher compared to the control.

Morphology analysis

Drugs with fermentation efficacy were treated and monitored for 70 days to assess the effects.

Immunofluorescence

When comparing hair follicle formation through Immunofluorescence (IF) analysis 10 days after drug treatment, increased expression of CTNNB1 was observed in the drug-treated organoids.

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