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  • High Precision
▶ Utilize our sensitive and accurate HET-CAM assay for precise irritancy evaluations without live animals.
  • Quick Results
▶ Achieve faster assessments with our efficient HET-CAM technique, streamlining the testing process.
  • Ethical Approach
▶ Our egg-based HET-CAM assay minimizes animal use, aligning with ethical research practices.

Organism
Egg
Product Type
Other
Tissue
Other
Disease

Applications

In vitro eye irritation test (HET-CAM)

The assessment of the degree of irritation is conducted by observingthe visual changes that occur when a test substance is applied to the chorioallantoic membrane of a fertilized.

Table of Contents

In vitro eye irritation test (HET-CAM)

OECD Test Guideline 432, known as the In Vitro 3T3 Neutral Red Uptake (NRU) Phototoxicity Test, is designed to identify the phototoxic potential of chemicals that become toxic only when exposed to light.
This in vitro assay uses mouse fibroblast cells (3T3 cells) to assess whether a chemical causes toxicity under exposure to a light source, simulating solar radiation.

Assay process

Sample preparation