A quantitative MDSC infiltration assay in a tumor organoid–stromal microenvironment for immuno-oncology candidate evaluation

This poster details a comprehensive methodology for evaluating the complex interactions between immune-suppressive cells and the tumor stroma:

• Innovative MDSC Modeling: Learn how we successfully differentiated THP-1 cells into functional MDSC-like cells using cytokine-driven conditions (G-CSF and IL-4), validated by flow cytometry and immune-regulatory gene signatures.
• Quantitative Infiltration Assays: Discover our Matrigel-coated transwell system (5-µm pore) that quantifies MDSC migration toward tumor organoids, CAFs, or a combination of both using imaging and FACS analysis.
• Integrated ADC Test Platform: Explore our workflow for evaluating ADCs (including targets like HER2, EGFR, and TROP2), which integrates internalisation analysis, 3D viability testing, and tissue-structure assessment.
• Microenvironment-Driven Insights: Understand how the presence of CAFs and the stromal microenvironment significantly impacts MDSC recruitment and drug response.
• Organ-Specific Toxicity Evaluation: See how we utilize iPSC-derived skin, intestinal, and retinal organoids to predict off-target toxicities, such as structural alterations caused by anti-NECTIN-4 ADCs.
• Precision Drug Screening: Review data from candidate MDSC-targeting agents (e.g., anti-STAT3, anti-IL-10) showing concentration-dependent inhibition of immune cell infiltration.