CRISPR Screens in 3D Gastric Organoids Reveal Gene Drivers of Cisplatin Response
Journal: Nature Communications
Author: Lo, YH., Horn, H.T., Huang, MF. et al., USA
Researchers used large-scale CRISPR screens in primary human 3D gastric organoids to map genes influencing cisplatin response. Combining CRISPR with single cell transcriptomics, they uncovered pathways like fucosylation affecting drug sensitivity and identified TAF6L as a regulator of recovery from cisplatin damage. The study demonstrates the power of organoid models for revealing therapeutic vulnerabilities in gastric cancer.
Comprehensive Single-Cell and Spatial Atlas Maps the Arabidopsis Life Cycle
Journal: Nature Plants
Author: Lee, T.A., Illouz-Eliaz, N., Nobori, T. et al. , USA/ UK
Scientists have created a single-nucleus and spatial transcriptomic atlas of Arabidopsis across 10 developmental stages, profiling over 400,000 nuclei from all major tissues. The atlas reveals extensive cell-type diversity, organ-specific heterogeneity, dynamic transcriptional programs, and new developmental regulators validated through functional studies. This resource offers a high-resolution framework for studying plant differentiation, growth, and environmental responses.
Radioactive Ion Beams Show Promise for Precision Image-Guided Cancer Therapy
Journal: Nature Physicsl
Author: Boscolo, D., Lovatti, G., Sokol, O. et al., Germany
Innovative “ORDER” method creates neural tube organoids with precise dorsal-ventral patterning by establishing opposing BMP and SHH gradients, closely mimicking human embryonic spinal cord development and enabling modeling of neural tube defects.
Mapping RNA-Protein Interactions and Gene Expression with MAPIT-seq
Journal: Nature Methods
Author: Cheng, QX., Xie, G., Zhang, X. et al., China
Researchers developed MAPIT-seq, a method that simultaneously maps RNA-binding protein (RBP) interactions and gene expression in situ, even at the single-cell level. The technique works across different RBPs, frozen tissues, and developmental contexts, offering new insights into post-transcriptional regulation and expanding multi-omics profiling for both basic and clinical research.
