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Regulatory T cell_main
Immune Cell

Regulatory T cell

7500€+
Immune Cell

Regulatory T cell

  • Regulatory T cells (Treg)” play a crucial role in maintaining immune homeostasis and tolerance by suppressing immune responses.
  • These lymphocytes inhibit T cell proliferation and cytokine production, thus preventing autoimmune reactions.
  • Our method involves evaluating the efficacy of an anticancer agent by treating a mixture of tumor organoids, cytotoxic T cells (CD8+ T cells), and regulatory T cells (Tregs) with the anticancer drug or drug candidate.

Price
Organism
Human
Product Type
Organoid + T cell + Regulatory T cell
Tissue
Adaptive
Disease

Applications

Cancer Organoid

Colorectal Cancer

Non-Small Cell Lung Cancer

Pancreatic Cancer

Breast Cancer

Cholangiocarcinoma

Gastric Cancer

Ovarian Cancer

Description

Table of Contents

Characteristics

A drug evaluation solution co-culturing Treg, T cells, and cancer organoids allows for the assessment of drugs that can regulate Treg.
This provides a drug evaluation solution capable of evaluating drugs that can modulate Treg.

Process

We aimed to develop tumor organoids with a tumor microenvironment and use them as a solution for drug evaluation.
For this, we created a system where tumor organoids, cytotoxic T cells, and regulatory T cells were co-cultured at specific ratios.
We activated T cells from PBMCs, then separately cultured and counted cytotoxic T cells and regulatory T cells.
Finally, after establishing the co-culture system at designated ratios, we measured the efficacy of immuno-oncology drugs.

R1 / T-cell

R1 / T-cell

Cell nr
Gated %
CD8+
47,600

All Events / Treg

CD4+CD5+ / Treg

Cell nr
Gated %
CD8+
CD25+
FoxP3
7,500
We compared the commonly used immuno-oncology drug atezolizumab with candidate.
When T cells alone or cytotoxic T cells and regulatory T cells were added, we examined the effect of each immuno-oncology drug.
Only in the case of co-culture with regulatory T cells, the efficacy of the drug decreased rapidly.
This is a result of regulatory T cells inducing T cell suppression during co-culture.
These results prove that our solution can most similarly simulate the tumor microenvironment consisting of tumor organoids, cytotoxic T cells and regulatory T cells.
Screen and accurately evaluate anti-cancer drugs targeting regulatory T cells using our validated solution that closely resembles the in vivo tumor microenvironment.

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