Holotomography

Platelet Research

Platelets are small, anucleate cells vital for hemostasis, inflammation, and immune response.
They undergo dynamic shape and size changes in response to stimuli, necessitating single-cell characterization of their morphological and biophysical properties.
While microscopy is crucial for platelet research, conventional techniques struggle with the small size and rapid activation of platelets, often requiring dyes that can affect their function.
Thus, there is a need for label-free methods to analyze live platelets' 3D morphology and biophysical characteristics.


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Applications

Holotomography

Immunology

Description

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Role of JAK2V617F mutation on the platelet phenotype.

(A) MIP of refractive index of representative WT and JAK2V617F platelets. (B) Representative three-dimensional surface rendering based on RI of WT and JAK2V617F platelets. (C) Quantitative analysis of biophysical properties (dry mass, mean RI) and morphological parameters (volume, sphericity, surface area and projected area). Statistical analysis: Mean values are displayed above each scatter plot. Data was analyzed using the non-parametric Mann-Whitney test for statistical significance where ns= non-significant, **p<0.01 and ****p<0.0001.; WT n=101, JAK2V617F n=102.

Reproduced from Ref. Tess A. Stanly et al., Frontiers in physiology (2020).