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Melan-A_main
2D Cell

Melan-A

Melan-A_main

  • Targeted Melanin Studies
▶ Utilize the Melan-A cell line to delve into melanin synthesis and regulation, critical for uncovering new pathways to reduce hyperpigmentation and enhance skin brightening.
  • Product Development and Testing
▶ Use Melan-A cells to rigorously test the efficacy of skin brightening agents, ensuring they target and modulate melanin production effectively.
  • Safety and Compatibility Testing
▶ Employ Melan-A cells to assess the safety and skin compatibility of new cosmetic products, making sure they are gentle yet effective for everyday use.
Organism
Product Type
Tissue
Disease

Applications

In vitro 3T3 NR (OECD TG 432)

Comparison of cytotoxicityinduced by chemicals under exposure to non-cytotoxic levels of light versus no exposure to light.

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Table of Contents
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Skin brightening

The skin brightening efficacy test is a thorough evaluation process designed to measure the effectiveness of skincare products in enhancing skin radiance.
It involves baseline assessments, consistent product application, and data collection using advanced analysis tools.

Assay process

  • Step 1_1: After culturing the cell line, it undergoes a stabilization process.
  • Step 1_2: Identify the concentration range for sample toxicity and establish the test concentration.
  • Step 2_1: Stabilize the cells by culturing them in a 48-well plate for approximately 1 day.
  • Step 2_2: After verifying the condition of the stabilized cells, treat them with the melanin production-stimulating hormone (a-MSH), either alone or simultaneously with the sample.
  • Step 2_3: Assess the whitening efficacy by comparing the amount of melanin production in the group treated with a-MSH alone and the group treated simultaneously with the sample
  • Step 3_1: Confirmation through repeated experiments, spanning from the initial cell seeding to the formation of melanin.
  • Step 3_2: Arranging raw data and generating quantitative or morphology data.

Read Out

Cell viability (% Neutral red uptake of dark controls)
  1. Microscopy data (cell morphology)
  2. Extracellular melanin content (%) data
  3. Intracellualr melanin content (%) data
Stimulation
  1. General screening stimulus: a-MSH (B16 cell line)
  2. In a light-induced melanin production model, the stimulus source can be replaceable with UVB
  3. Efficacy screening is feasible without irritants when utilizing cell lines other than the B16 cell line (high reproducibility).
Cell line
  1. Murine melanoma cell line : B16F10 , Melan-A
  2. Human melanoma cell lin : MNT-1
  3. Primary human melanocytes

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