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iPSC Generation_main
Research Service

iPSC Generation

iPSC Generation_main

  • Quality Control and Extensive Experience
▶ With over 1000 iPSC lines generated, we ensure top-quality control through rigorous testing, including RNA reprogramming vector clearance and mycoplasma testing.
  • Rapid Custom Constructs
▶ Quickly obtain stem cell reporter custom constructs with our pipette-free service. Simply email us your gene sequence, and we’ll synthesize and clone it into any of our inducible vectors for minimal cost.
  • Safe and Standardized Reprogramming
▶ Utilizing Sendai virus vectors, we produce transgene-free iPSCs, providing a safer, non-integrating alternative to traditional methods. Our standard package includes the generation of three different iPSC lines per individual and comprehensive pluripotency characterization.

Organism
Human
Product Type
iPSC
Tissue
Kreatinocytes, Urine, Blood, PBMCs
Disease

Applications

iPSC Generation

iPSC lines from patient donor cells ensuring safety and quality through standardized protocols and thorough testing.

iPSC Differentiation

Our iPSC differentiation service offers a comprehensive solution for researchers seeking to explore a diverserange of cell types derived from induced pluripotent stem cells (iPSCs).

Disease Modeling

Utilizing our iPSC technology, we create disease-specific cell models that faithfully mimic human disease conditions.

Drug Discovery

Lambda assists in your drug discovery endeavors by providing a wide range of services, spanning from initial discovery and high-throughput screening to toxicity testing using iPSC-derived cells.

Table of Contents

Application of iPSC

Induced pluripotent stem cells (iPSCs) are created by reprogramming somatic cells to gain pluripotency, allowing them to differentiate into any cell type in the body.
This capability is crucial for studying cellular functions, creating disease models, and developing patient-specific therapies.

iPSC generation using non-integrating system

We utilize a variety of cells, including PBMC (peripheral blood mononuclear cells) and fibroblasts, to produce iPSCs.
Notably, our iPSC production method employs a non-integrating approach, ensuring both safety and efficiency.
By generating iPSCs without genetic modification, we obtain more natural and stable stem cells.

Undifferentiated marker analysis

We rigorously check the presence of key pluripotency markers via flow cytometry and immunocytochemistry (ICC) to ensure that iPSCs remain undifferentiated until needed for your specific research purposes.

Three germ layer differentiation potential analysis

Verifying the true pluripotency of iPSCs (induced pluripotent stem cells) is a critical step in research.
The confirmation of trilineage differentiation involves assessing the ability of iPSCs to differentiate into the three germ layers: endoderm, mesoderm, and ectoderm.
This evaluation is conducted through teratoma formation and spontaneous differentiation into the three germ layers.

Mycoplasma test

To guarantee safety of our cell cultures, we perform thorough mycoplasma contamination testing.
This ensures that our iPSCs are free from contaminants that could compromise your research.

Vector clearance test

We confirm the removal of reprogramming vectors to prevent any residual vector sequences from affecting the iPSCs

STR analysis

STR analysis is used to confirm that the original somatic cells and the reprogrammed iPSCs share the same genetic profile.
This ensures that the cells have been correctly reprogrammed.

Karyotype analysis

Through karyotype analysis, it was confirmed that there were no chromosomal mutations in the iPSCs produced.

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