2D Cell

BALB 3T3 OECD TG 432

Consistency and Reliability
BALB 3T3 cells offer high reproducibility and consistent outcomes in biological experiments, enhancing the reliability of research results.
Versatile Research Applications
These cells are extensively used in a variety of biological and medical research areas, including cancer research, toxicity testing, and drug response analysis.
Their versatility broadens the scope of research possibilities.
Genetic Stability
BALB 3T3 cells maintain stable characteristics over long periods due to low genetic variability, making them ideal for long-term studies and repetitive experiments.


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Applications

OECD

Phototoxicity Test

Description

OECD Test Guideline 432: In Vitro 3T3 NRU Phototoxicity Test

OECD Test Guideline 432, known as the In Vitro 3T3 Neutral Red Uptake (NRU) Phototoxicity Test, is designed to identify the phototoxic potential of chemicals that become toxic only when exposed to light.
This in vitro assay uses mouse fibroblast cells (3T3 cells) to assess whether a chemical causes toxicity under exposure to a light source, simulating solar radiation.

Purpose of the test

The test aims to determine if a substance is likely to cause damage to the skin or eyes when exposed to sunlight or other light sources, helping to ensure the safety of chemicals and products such as cosmetics, pharmaceuticals, and industrial substances.

Test Process

1. Preparation of RhE Models

Cultivate human epidermal cells to form a multi-layered, differentiated model that replicates human epidermis.

2. Chemical Application

Apply the test substances directly onto the RhE model.

3. Incubation

Allow the chemicals to interact with the epidermis for a specified duration, typically about 15 to 60 minutes, depending on the protocol.

4. Post-exposure Treatment

Rinse the chemicals off the RhE model and apply a viability dye such as MTT, which will be metabolized by living cells.

5. Viability Measurement

Measure the conversion of the dye to formazan by viable cells, which is quantified using a colorimetric assay to assess cell viability.

6. Data Analysis

Analyze the results to determine the reduction in cell viability compared to untreated control samples, indicating the irritant potential of the test substance.

7. Reporting

Prepare detailed reports including the methods, results, and conclusions for regulatory and safety assessment purposes.