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Artificial Cornea(RhCE) OECD TG 492

Alternative to Animal Testing
The RhCE test uses reconstructed human cornea tissue, providing an ethical, reliable alternative to animal testing by closely simulating human physiological responses.
Fast and Efficient Results
This method delivers results quickly, reducing research timelines and costs. It uses the MTT assay to objectively assess irritation through cell viability measurements.
Internationally Trusted Standards
Recognized under OECD TG 492 and aligned with UN GHS, the RhCE test is a globally accepted method for reliably assessing eye irritation, aiding regulatory compliance worldwide.


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Organism	–
Product Type	–
Tissue	–
Disease	–

Applications

OECD

Eye Irritation

KTR (Korea Testing & Research Institute)

Testimonials

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KTR established Korea's first Animal Alternative Testing Center and has built related facilities in the biopharmaceutical sector to provide an innovative research environment. Currently, toxicity evaluations required in the drug...

Description

In vitro eye irritation test (OECD TG 492)

Reconstructed human Cornea-like Epithelium (RhCE) test method for identifying chemicals not requiring classification and labelling for eye irritation or serious eyedamage.

Eye irritation test – Artificial cornea

Using the human corneal-like epithelium (RhCE) model, which closely mimics the histological, morphological, biochemical, and physiological characteristics of the human corneal epithelium, the assessment of eye irritation (UN GHS Category 2) or severe eye damage (UN GHS Category 1) is conducted according to UN GHS guidelines.
This evaluation is utilized to assess the toxicity of testsubstances (compounds and mixtures) that do not require classification andlabeling for UN GHS Category 1 (UN GHS No Category).

Test Process

Step 01

Preculture
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Medium 0.9ml 37°C, 5% CO2, 22±2hours

Step 02

Test sample treatment
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Liquide: 40μl. 10min Solid: DPBS 40μl + 40mg, 37°C, 5% CO2, 3hours

Step 03

Wash
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Washing the inside and outside of the human cornea model with DPBS

Step 04

Post-culture
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Medium 0.9ml 37°C, 5% CO2, 16±1hour

Step 05

WST-1 assay
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WST-1 solution 300μl (insert inside 100μl, insert outside 200μl) 37°C, 5% CO2, 3hours±5min

Step 06

Absorbance Measurement
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Supernant 200μl / well(96 well plate) 450nm

Artificial Cornea(RhCE) OECD TG 492

Artificial Cornea(RhCE) OECD TG 492

Testimonials

Advancing Drug Development: KTR and Lambda Biologics Unite for GLP-Certified, Organoid-Based Alternative Testing

Description

Table of Contents

In vitro eye irritation test (OECD TG 492)

Eye irritation test – Artificial cornea

Test Process

Read out

Solutions

Insights

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Artificial Cornea(RhCE) OECD TG 492

Artificial Cornea(RhCE) OECD TG 492

Testimonials

Advancing Drug Development: KTR and Lambda Biologics Unite for GLP-Certified, Organoid-Based Alternative Testing

Description

Table of Contents

In vitro eye irritation test (OECD TG 492)

Eye irritation test – Artificial cornea

Test Process

Read out

Solutions

Insights

MyLab

About us

Contact

Leave a message

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