Skin brightening

In vitro

Lambda offers a highly mimetic in vitro skin model for conducting various skin-related tests.
We provide 11 efficacy testing services for skin, leveraging our advanced skin mimicry technology.

€1.920+

€1.920+

€1.920+

€640+

€1.920+

In vitro eye irritation

(OECD TG 437)

In vitro eye irritation

(HET-CAM Assay)

In vitro 3T3 NRU

(OECD TG 432)

Preparing

Skin regeneration

Preparing

Skin barrier

Preparing

Anti-irritation

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The skin brightening efficacy test is a thorough evaluation process designed to measure the effectiveness of skincare products in enhancing skin radiance.
It involves baseline assessments, consistent product application, and data collection using advanced analysis tools.

Anti-melanogenesis assay

Assess the samples’ capability to inhibit melanin production or secretion by utilizing melanocytes, the cells that regulate skin pigmentation.

Starting cost

€ 0 +

Assay process

Step 1_1: After culturing the cell line, it undergoes a stabilization process.
Step 1_2: Identify the concentration range for sample toxicity and establish the test concentration.

Step 2_1: Stabilize the cells by culturing them in a 48-well plate for approximately 1 day.
Step 2_2: After verifying the condition of the stabilized cells, treat them with the melanin production-stimulating hormone (a-MSH), either alone or simultaneously with the sample.
Step 2_3: Assess the whitening efficacy by comparing the amount of melanin production in the group treated with a-MSH alone and the group treated simultaneously with the sample

Step 3_1: Confirmation through repeated experiments, spanning from the initial cell seeding to the formation of melanin.
Step 3_2: Arranging raw data and generating quantitative or morphology data.

Readout

Microscopy data (cell morphology)
Extracellular melanin content (%) data
Intracellualr melanin content (%) data

Stimulation

General screening stimulus: a-MSH (B16 cell line)
In a light-induced melanin production model, the stimulus source can be replaceable with UVB
Efficacy screening is feasible without irritants when utilizing cell lines other than the B16 cell line (high reproducibility).

Cell line

Murine melanoma cell line : B16F10 , Melan-A
Human melanoma cell lin : MNT-1
Primary human melanocytes

In vitro

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