Salivary gland organoids exhibit diverse cell types, replicating the tissue’s architecture, including acinar and ductal structures.
They showcase secretory function and ductal networks, crucial for studying gland physiology.
Valuable for drug testing, these organoids help assess pharmaceutical effects and study potential treatments.
As disease models, they aid in understanding conditions like Sjögren’s syndrome and support therapeutic development.
Salivary gland organoids offer a scalable method for in vitro studies, advancing our understanding of oral health and potential clinical applications.
Salivary gland organoid
Histological analysis results (H&E, PAS) revealed an increase in cellular components and an observed increase in cells producing neutrophil mucin under organoid differentiation conditions. Therefore, it was confirmed that the similarity with the tissue is more pronounced during differentiation induction.
In the organoids subjected to differentiation conditions, there was an increased expression of acinar cell markers (AQP5, AMY), and the expression of ductal cell markers (CK7, CK19) exhibited a polarized pattern, resembling the characteristics of actual ducts.
The ability to secrete saliva was verified by observing the response of calcium accumulation upon stimulation with the cholinergic agonist carbachol.
