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Home » AFS » PhenoCycler
Spatial Biology

PhenoCycler

9500€+

PhenoCycler

PhenoCycler enables high-plex spatial protein profiling on a single tissue section, providing detailed spatial insights into cellular composition and expression patterns. Using DNA-barcoded antibodies, up to 100 protein markers can be analyzed with precise single-cell segmentation and quantitative resolution, revealing cellular phenotypes and interactions within complex tissues.

Price
9500€+
Organism
Human
Product Type
Organoid, Tissue
Tissue
Refer to our panel for detailed insights
Disease

Applications

Spatial Biology

Protein Solution

Professor Lee Chang-seok Eulji University
Customer insight

Advancing K-Beauty with Skin Organoids: A Next-Generation Platform for Non-Animal Testing and High-Precision Cosmetic Innovation

With the global rise of K-beauty, the cosmetics industry continues to grow steadily. Since the ban on animal testing for cosmetics in Korea in 2017, various alternative testing methods have...

Tomocube (Spatial)
Customer insight

HT-X1: A Label-Free Imaging Breakthrough for Organoid-Based Disease Modeling and Drug Screening

Traditional microscopy methods often require fluorescent labeling to analyze cellular structures, which can be time-consuming and invasive. In contrast, our HT-X1 system allows for high-resolution visualization of cellular morphology without...

Seoul National University College of Medicine
Customer insight

Pioneering Spatial Protein Analysis in Korea: Advancing Clinical Pathology with Lambda Biologics’ Support

Traditional protein analysis has primarily focused on quantifying expression levels within tissue samples. However, recent advances in spatial analysis techniques have shifted attention toward evaluating not only expression levels, but...

K Research Institute
Customer insight

ODISEI-Gut Platform Reveals Immune-Boosting Potential of Kimchi-Derived Bacterial Strain

Among the many fermented foods we consume, kimchi is particularly known for containing a diverse range of lactic acid bacteria, which are believed to influence the activation of immune cells...

Bundang Jesaeng General Hospital
Customer insight

Multiplex Marker Analysis Enhances Research Efficiency with 31-Marker Detection on a Single Slide

We conducted a study focused on identifying disease-related markers using patient-derived tissue samples. However, traditional methods limited our ability to analyze multiple candidate markers simultaneously, and the limited availability of...

Description

PhenoCycler

PhenoCycler (formerly CODEX) was developed to overcome the limitations of existing IHC. Multiplex-IHC is an experimental method that can confirm the expression of more than 100 biomarkers on one tissue slide. It is possible to check the expression of various biomarkers on a single tissue slide and conduct comprehensive research on cell composition, cell function and state, and cell-cell interaction. CODEX analysis is performed using DNA barcoding and uses antibodies conjugated to unique oligonucleotide sequences. By using a target specific barcode called dye-labelled reporter, it is possible to stain more than 100 biomarkers by repeating the labeling cycle by dyeing three colors of fluorescence in one cycle.
PhenoCycler
  • Staining more than 100 biomarkers on tissue slides 1 chapter.
  • Mapping of Millions of Cells Through Diverse Panel Combinations.
  • Single cell resolution analysis.
  • Cell phenotyping using marker staining combinations.
  • Cellular Neighborhood Analysis Utilizing Spatial Information of Cells.

Process

Immune profiling panels type

Immune panel - 31 markers
IO60 Panel
Immune cell type core
Immune activation
Tissue architecture
Lymphocyte profiling
Myeloid profiling
Proliferation
Tumor-specific marker

Readout

31 markers (charged slide)
To identify the mechanism of a new target, acquire spatial information by simultaneously staining 31 markers in PDOX tissue.
Simultaneous staining of 31 markers in tumor tissue
Identification of treatmentmechanism through spatial analysis of cell types
Identifying immunological mechanisms underlying the efficacy of anticancer immunotherapy and discovering biomarker candidates through theapplication of spatial biology

Unlock High-Dimensional Spatial Insights

Discover cellular phenotypes, spatial interactions, and tissue architecture at single-cell resolution with Lambda Biologics PhenoCycler service. Whether you’re studying tumor microenvironments, immune landscapes, or complex tissues, our team is ready to support your research goals.

FAQs

PhenoCycler is compatible with a wide range of samples, including human tissue sections, FFPE or fresh-frozen samples, and complex 3D tissue models. Our team will assess sample suitability during project consultation.

Using DNA-barcoded antibodies, the PhenoCycler platform enables simultaneous analysis of up to 100 protein markers on a single tissue section, without compromising spatial resolution.

PhenoCycler delivers single-cell resolution, allowing precise cell segmentation and quantitative measurement of protein expression while preserving native tissue architecture.

This service is ideal for studies focused on tumor microenvironment characterization, immune profiling, cell–cell interaction analysis, and biomarker discovery, particularly in oncology and immunology research.

Yes. Lambda Biologics offers end-to-end support, including image processing, cell segmentation, marker quantification, spatial analysis, and data interpretation tailored to your research objectives.

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