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Viral Vector Cloning

Our viral vector technology is revolutionizing research in virology, particularly in the study of nerve cells where traditional gene transfer methods fall short.
This cutting-edge tool, endorsed by esteemed publications like Science (2010) and others (2012), facilitates a myriad of experimental approaches.
We offer a range of recombinant viruses, including adenovirus, AAV (Adeno-Associated Virus), and lentivirus, supplied in high concentrations ideal for in vivo experiments.
These viruses empower researchers with precise control over gene expression for over 50 types of genes.
Moreover, our Cre-lox conditional gene silencing shRNA library, available with Cre On or Cre Off systems, expands research possibilities.
For targeting new genes, we provide Cre On and Cre Off shRNAs alongside our robust Cre On and Cre Off system viral vectors, ensuring comprehensive support for your research endeavors.
Cre-On and Cre-Off shRNAs targeting new genes are also available.
The Cre-On and Cre-Off systems are available for viral vector support. There are two types of vectors below.

Starting cost
€ 0 +

Viral Vector Cloning

Viral Vector Cloning

Our viral vector technology is revolutionizing research in virology, particularly in the study of nerve cells where traditional gene transfer methods fall short.
This cutting-edge tool, endorsed by esteemed publications like Science (2010) and others (2012), facilitates a myriad of experimental approaches.
We offer a range of recombinant viruses, including adenovirus, AAV (Adeno-Associated Virus), and lentivirus, supplied in high concentrations ideal for in vivo experiments.
These viruses empower researchers with precise control over gene expression for over 50 types of genes.
Moreover, our Cre-lox conditional gene silencing shRNA library, available with Cre On or Cre Off systems, expands research possibilities.
For targeting new genes, we provide Cre On and Cre Off shRNAs alongside our robust Cre On and Cre Off system viral vectors, ensuring comprehensive support for your research endeavors.
Cre-On and Cre-Off shRNAs targeting new genes are also available.
The Cre-On and Cre-Off systems are available for viral vector support. There are two types of vectors below.

Eye Irritation test – HET-CAM assay

Cre-On shRNA

Cre-Off shRNA

Starting cost
€ 0 +

Supply Service

iPSC Differentiation

Virus

Lentivirus

AAV

Adenovirus

Amount*(μl)

100 / 200 / 1,000

*Titer is above 1012/ml

Customized virus

Service

Cell type specific shRNA production and packaging

Gene cloning and packaging

Gene cloning only (One step)

Plasmid maxi prep.

Types of recombinant viruses available for service

Virus

Lentivirus

Insert / Vector (kb)

7/8

Target cells

Dividing or non-dividing

Remark

  • Low immune response
  • Low titer

AAV

3/4.8

Dividing or non-dividing

  • Non-pathogenic
  • Small insert size

Adenovirus

8/36

Dividing or non-dividing

  • Hightiter & efficiency
  • Immunogenic

AAV-CreVirus List

AAV-CMV-cre-GFP

AAV-CMV-mCherry-cre

AAV-CMV-mCherry-IRES-cre

AAV-CaMKIIa-cre

AAV-CaMKIIa-nls-cre-2A-EGFP

AAV-CaMKIIa-cre-mCherry
AAV-EF1a-Cre
AAV-EF1a-EGFP-IRES-cre
AAV-GFAP-cre
AAV-GFAP-EGFP-cre
AAV-GFAP-mCherry-cre
AAV-hvGAT-cre
AAV-hSyn-cre
AAV-hSyn-nls-cre-IRES-TdTomato

Cre Dependent Vector

AAV-CaMKIIa-DIO-EGFP
AAV-CaMKIIa-DIO-mCherry
AAV-CaMKIIa-DIO-EYFP
AAV-EF1a-DIO-EGFP
AAV-EF1a-DIO-mCherry
AAV-EF1a-DIO-EYFP
AAV-CMV-DIO-EGFP
AAV-CMV-DIO-mCherry
AAV-CMV-DIO-EYFP
AAV-hSyn-DIO-EGFP
AAV-hSyn-DIO-mCherry
AAV-GFAP104-DIO-EGFP
AAV-GFAP104-DIO-mCherry

GCaMP/RCaMP

AAV-hSyn-jGCaMP7b
AAV-hSyn-Flex-GCaMP6f
AAV-hSyn-GCaMP6f-P2A-nls-dTomato
AAV-hSyn-mRuby2-GSG-P2A-GCaMP6m
AAV-hSyn-GCaMP6s-P2A-nls-dTomato
AAV-EF1a-DIO-GCaMP6s-P2A-nls-dTomato
AAV-EF1a-DIO-GCaMP6f-P2A-nls-dTomato
AAV-EF1a-FAS-GCaMP6f-P2A-dTomto
AAV-EF1a-FAS-GCaMP6s-P2A-dTomato
AAV-CaMKIIa-GCaMP6s-P2A-nls-dTomato
AAV-CaMKIIa-GCaMP6f-P2A-nls-dTomato
AAV-CMV-GCaMP6s-P2A-nls-dTomato
AAV-GFAP-GCaMP6f
AAV-GFAP104-GCaMP6S-P2A-nls-dTomato
AAV-GFAP104-GGCaMP6f
AAV-GFAP104-GCaMP6f-P2A-nls-dTomato
AAV-hSyn-jRCaMP1a
AAV-EF1a-jRCaMP1a
AAV-CaMKIIa-jRCaMP1a
AAV-Ef1a-FAS-jRCaMP1a
AAV-Ef1a-FAS-jRCaMP1b

GECO

AAV-AND-GECO1
AAV-hSyn-REX-GECO1
AAV-hSyn-REX-GECO0.9
AAV-hSyn-NES-jRGECO1a
AAV-hSyn-NES-jRGECO1b
AAV-hSyn-Flex-NES-jRGECO1a
AAV-CAG-Flex-NES-jRGECO1b

hM4D(Gi)/hM3D(Gq)/rM3D(Gs)

AAV-EF1a-hM4D(Gi)-mCherry
AAV-EF1a-DIO-hM4D(Gi)-mCherry
AAV-CaMKIIa-hM4D(Gi)-mCherry
AAV-hSyn-hM4D(Gi)-mCherry
AAV-hSyn-DIO-hM4D(Gi)-mCherry
AAV-hSyn-DIO-HA-hM4D(Gi)-IRES-mCitrine
AAV-GFAP-hM4D(Gi)-mCherry
AAV-CD68-hM4D(Gi)-mCherry
AAV-EF1a-hM3D(Gq)-mCherry
AAV-EF1a-hM3D(Gq)-EYFP
AAV-EF1a-DIO-hM3D(Gq)-mCherry
AAV-CaMKIIa-hM3D(Gq)-mCherry
AAV-hSyn-HA-hM3D(Gq)-mCherry
AAV-hSyn-DIO-hM3D(Gq)-mCherry
AAV-hSyn-DIO-HA-hM3D(Gq)-IRES-mCitri
AAV-GFAP-hM3D(Gq)-mCherry
AAV-hCD68-hM3D(Gq)-mCherry
AAV-CaMKIIa-HA-rM3D(Gs)-IRES-mCitrine
AAV-hSyn-DIO-rM3D(Gs)-mCherry
AAV-hSyn-DIO-HA-rM3D(Gs)-IRES-mCitrine

hChR2(H134R)

AAV-EF1a-hChR2(H134R)-EGFP
AAV-EF1a-hChR2(H134R)-mCherry
AAV-EF1a-hChR2(H134R)-EYFP
AAV-EF1a-DF-hChR2(H134R)-EYFP
AAV-EF1a-DF-hChR2(H134R)-mCherry
AAV-EF1a-FAS-hChR2(H134R)-mCherry
AAV-CMV-hChR2(H134R)-EGFP
AAV-CMV-hChR2(H134R)-EYFP
AAV-CaMKIIa-hChR2(H134R)-mCherry
AAV-CaMKIIa-hChR2(H134R)-EYFP
AAV-CaMKIIa-hChR2(C128S/D156A)-mCherry
AAV-CaMKIIa-hChR2(E123A)-mCherry
AAV-hSyn-hChR2(H134R)-mCherry
AAV-hSyn-hChR2(H134R)-EYFP
AAV-hSyn-hChR2(H134R)-EYFP
AAV-hSynCon/FoffhChR2(H134R)-EYFP
AAV-hSynCoff/FoohChR2(H134R)-EYFP
AAV-GFAP-hChR2(H134R)-mCherry
AAV-GFAP104-hChR2(H134R)-EYFP
AAV-hALDH1L1-hChR2(H134R)-EGFP
AAV-hALDH1L1-hChR2(H134R)-mCherry

eNpHR3.0

AAV-EF1a-eNpHR3.0-EYFP
AAV-EF1a-DIO-eNpHR3.0-EYFP
AAV-CMV-eNpHR3.0-EYFP
AAV-CaMKIIa-eNpHR3.0-EYFP
AAV-hSyn-eNpHR3.0-EYFP
AAV-GFAP104-eNpHR3.0-EYFP

eArchT3.0

AAV-EF1a-eArchT3.0-P2A-GFP
AAV-CMV-eArchT3.0-P2A-EGFP
AAV-CaMKIIa-eArch3.0-EYFP
AAV-CaMKIIa-eArChT3.0-P2A-EGFP
AAV-hSyn-eArchT3.0-EYFP
AAV-hSyn-eArchT3.0-P2A-EGFP
AAV-GFAP104-eArchT3.0-P2A-EGFP
AAV-hALDH1L1-eArchT3.0-P2A-EGFP

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