Individualized patient tumor organoids faithfully preserve human brain tumor ecosystems and predict patient response to therapy
Journal: Cell Stem Cell
Author: Tianping Peng et al., China
Individualized Patient Tumor Organoids (IPTOs) enable efficient brain tumor modeling by combining cerebral organoids and tumor explants, maintaining tumor heterogeneity and predicting treatment responses.
Sustained chromosomal passenger complex activity preserves the pluripotency of human embryonic carcinoma cells
Journal: Science Signaling
Author: Takaaki Tsunematsu et al., Japan
A study reveals CPC stability in embryonic carcinoma cells depends on low APC/CCdh1 activity. CPC disruption triggers cell differentiation, while CPC inactivation activates EMT, indicating its role in stem cell maintenance.
In vivo expansion of gene-targeted hepatocytes through transient inhibition of an essential gene
Journal: Science Translational Medicine
Author: Marco De Giorgi et al., USA
An in vivo gene editing method ‘Repair Drive’ enables targeted hepatocyte expansion by combining essential gene knockdown with therapeutic transgene delivery, achieving 25% correction without toxicity in mouse liver.
BPS2025 – A novel object-based spatial analysis pipeline captures structural heterogeneities across all length scales contained within a biological sample
Journal: Biophysical Journal
Author: Nathan Rothacker et al., USA
High-resolution imaging workflow combines confocal microscopy and spatial analysis to assess tissue heterogeneity at multiple scales. Method validated on cardiac tissue enables segmentation, protein clustering analysis, and generation of structural heatmaps.
