CODEX

Ultrahigh-Multiplex Spatial Profiling Solution

Starting cost

€ 0 +

€15.380+

CODEX

€4.480+

OPAL

Visium

€8.200+

Preparing

GeoMX

€10.890+

Preparing

Hyperion

€3.200+

Request a quote

CODEX

CODEX was developed to overcome the limitations of existing IHC.
Multiplex IHC is an experimental method that can confirm the expression of more than 100 biomarkers on one tissue slide.
It is possible to check the expression of various biomarkers on a single tissue slide and conduct comprehensive research on cell composition, cell function and state, and cell-cell interaction.
CODEX analysis is performed using DNA barcoding and uses antibodies conjugated to unique oligonucleotide sequences.
By using a target specific barcode called dye-labelled reporter, it is possible to stain more than 100 biomarkers by repeating the labeling cycle by dyeing three colors of fluorescence in one cycle.

CODEX

Staining more than 100 biomarkers on tissue slides 1 chapter.
Mapping of Millions of Cells Through Diverse Panel Combinations.
Single cell resolution analysis.
Cell phenotyping using marker staining combinations.
Cellular Neighborhood Analysis Utilizing Spatial Information of Cells.

Process

Staining

2 days

FFPE or FF tissue section
Antigen Unmaking
Tissue staining
Primary antibody incubation
Reporter plate preparation
PCF run

Image

1-3 days per slide

Continuous speed improvements
Workflow simplification
Consistent imaging methods

Analyze

4 months

Proprietary data compression
Solutions serve every user need
Consistent image analysis methods

Step 1 : Sample preparation
Step 2 : Tissue staining (primary antibody)
Step 3 : Reporter plate preparation
Step 4 : PCF run
Step 5 : Data analysis

Immune profiling panels type

Immune panel – 31 markers

STEP core panel

CD4

CD68

CD20

CD11c

CD8

HLA-DR

CD3e

CD44

CD45

HLA-A

CD14

Ki67

Pan-CK

CD56

CD45RO

Helper T cells

Macrophages

B cells

Dendritic cells

Cytotoxic T cells

APCs (MHC II)

T cells

Activated T cells

Immune cells

MHC I

Monocytes

Proliferating cells

Tumor cells

NK cells

Memory T cells

Advanced Immune module

CD163

FoxP3

Granzyme B

CD21

M2 Macrophages

Regulatory T cells

Activated T cells / NK cells

Dendritic cells, mature B cells, FDCs

Immune activation module

PD-1

LAG-3

ICOS

PD-L1

IDO1

Checkpoint receptor

Checkpoint ligand

Multifunctional/Immune inhibitory

Structural module

E-cadherin

SMA

Vimentin

Collagen IV

CD31

CD34

Podoplanin

Epithelial cells

Vascular, Fibroblasts, Smooth muscle

Cyto-structures

Extracellular matrix

Vascular structures

Lymphatics

Readout

31markers (charged slide)

PDOX BTLA

PDOX HVEM

To identify the mechanism of a new target, acquire spatial information by simultaneously staining 31 markers in PDOX tissue.

Simultaneous staining of 31 markers in tumor tissue

IgG control

Cell types

IgG control

Sub types

Anti-Target

Cell types

Anti-Target

Sub types

Identification of treatment mechanism through spatial analysis of cell types

IgG control

Cell types

IgG control

Sub types

Anti-BTLA

Cell types

Anti-BTLA

Sub types

IgG control

Cell types

Total = 236,688

IgG control

Sub types

CD44-, 21.9%
HLA-A-, 14,4%

Total = 236,688

Anti-Target

Cell types

Total = 188,595

Anti-Target

Sub types

CD44-, 54.2%
HLA-A-, 26,6%

Total = 188,595

IgG

Anti-Target 1

Anti-Target 2

Target 2 = Binging partner for Target 1

% of positive cells

E-cadherin

% of positive cells

CD44

% of positive cells

CD8

Identifying immunological mechanisms underlying the efficacy of anticancer immunotherapy and discovering biomarker candidates through theapplication of spatial biology